Bioacoustics of Izecksohniella puri

Diagnosis. Head with three ocelli, forming an equilateral triangle. Male forewing reduced ( Fig. 1 B); forewing without glandular hair, stridulatory vein and glandular border. Tympanum present in internal face of tibia I. Tibia III with four internal dorsal spurs (the first and fourth smaller than the second and third) and four external, (the first smaller than the others, that are similar). Three internal apical spurs, the first is half of the size of the others and three external apical spurs, first and third smaller than second. Metanotal gland absent. Male terminalia: with phallic glands; pseudepiphallic arms long, curved ventral-apically, fining gradually, with teeth on all extension ( Fig. 6 A); pseudepiphallic paramere 2 cut ( Fig. 6 A), teethed ventrally ( Fig. 6 B); pseudepiphallic paramere 1 in “L” shaped, with small denticles on the internal side; female copulatory papilla sclerotized, conical shaped ( Fig. 7 A-C). Marliella titai Mews & Mól sp. nov. ( Figs.1 B, 6 and 7)

Male. Head: evenly medium brown. Vertex dark brown with two light medium stripe. Fastigium dark brown; gena medium brown with dark brown patch below to the eyes. Clypeus, labrum medium brown, labial palpi light brown. Maxillary palpi: light brown of the first to the third segment, fourth segment medium brown, fifth segment dark brown, apex either narrow as the base, resembling a scythe. Antennae: medium brown, scape and pedicel medium brown; flagellum medium brown with white in some segments. Pronotum: medium brown, lateral lobes rectangular dark brown. Metanotum without glands, but with two broad humps on its anterior-lateral borders and two smaller callus-shaped ones between then. Forewings covering one abdominal tergite, medium brown, apical border pale yellow, with lateral field reduced; without glandular hairs, inflated border, stridulatory vein and specialized veins ( Fig. 1 B). Abdominal tergites medium brown; abdominal sternites pale yellow. Legs with thick dark hair. Coxa and trochanter light brown with medium brawn marks. Femur I medium brown; femur II medium brown; femur III: proximal portion medium brown with transversal pale yellow stripe; distal portion dark brown. Tibia I medium brown; tibia II medium brown; tibia III medium brown, serrulated: 22 spines on inner and 24 on outer margin, among dorsal spurs. Tarsi I light brown with eight spines in outer dorsal margin and six spines in inner dorsal margin; Tarsi II light brown and III medium brown. Cerci light brown. Terminalia: Phallic gland, secretor duct and orifice present. ( Fig. 6 A-C). Pseudepiphallic arms: the left crosses (raisin on) on the right. Pseudepiphallic arms long curved ventral-apically, fining gradually, with teeth on all extension ( Fig. 6 A). Pseudepiphallic paramere 2 cut, membranous in dorsal side ( Fig. 6 A); teethed ventrally ( Fig. 6 B). Pseudepiphallic paramere 1 in “L” shaped, with small denticles on the internal side. Ectophallic fold reaching the base of parameres ( Fig. 6 A, B). Ectophallic apodeme: base curved for the center of the genitalia, apex with two apical projections that reach the middle of the parameres, giving to the apodeme, with "H"-shaped. Endophallus small. Rami short ( Fig. 6 B, C). Supra-anal plate medium brown on the base and dark brown apex, longer than wide ( Fig. 6 D). Subgenital plate light brown on the base and medium brown on the apex, bilobed apically ( Fig. 6 E). Female. Coloration as adult male. Terminalia: copulatory papilla conical dorsum-ventrally ( Fig. 7 A-C); base and apex membranous in dorsal side ( Fig. 7 A), sclerotized in ventral side ( Fig. 7 B), laterally cylindrical, continuing to distal extremity ( Fig. 7 C). Median valve of ovipositor as in figure 7D. Supra-anal plate wider than long with rounded apex ( Fig. 7 E). Subgenital plate narrow and convex, with pronounced medium depression ( Fig. 7 F). Measurements in mm . (Male (n=10) min. – max. values, followed by female (n=10) min. – max. values): body length: 5.40–5.65, 4.90–6.00; head width: 1.10–1.20, 1.15–1.30; intra-ocular distance: 0.60– 0.70, 0.60–1.20; pronotum length: 0.70–0.85, 0.90–1.30; pronotum width: 1.20–1.40, 1.40–1.60; femur III length: 4.50–4.85, 4.00–5.20; tibia III length: 4.80–5.20, 4.00–5.00; male wing length: 0.90–1.05; male wing width: 0.90–1.00; female ovipositor length: 0.70–2.10.

Description . Measurements in Table 1 . Dorsal coloration dark, contrasting to pale ventral coloration. Head homogenously reddish, distinct from the darker body color ( Fig. 1 A, B, Fig. 2 F). Three circular occelli forming a triangle, the inferior one between antennae and the other shortly above. Clypeus and labrum whitish, with short bristles. Scape longer than wide, reddish. Antennal flagellum with dark alternated with white groups of antennomeres, white groups never longer than dark ones ( Fig. 3 A). Scape and first five antennomeres dark, followed by a white, six dark, two white, four dark, two white, eight dark and four white. In the sequence, there is a color pattern variation between individuals, but always alternating longer dark and shorter white portions. First three segments of maxillary palps pale, and the two apical ones darker; first two basal segments smaller than the other, and last segment truncated at apex. Anterior lateral corners of pronotum folded in oblique angle. External surface of femur III with longitudinal color pattern as in Figure 3 B. Foretibiae without tympanum, with one pair of movable apical ventral spurs. Tibiae I and II without serrulation nor dorsal spurs. Tibia III with dorsal serrulation, four pairs of dorsal, movable, spurs, and three pairs of apical, movable spurs, median spurs longer than ventral and dorsal on each face, and external spurs smaller than internal. All tarsi with first tarsomere largest, and second smallest, with curved tarsal claws. First two tarsomeres of tarsi I and II with two raws of ventral serrulations and a pair of apical spurs, while there are no ventral serrulations on tarsus III, and its first tarsomere present two rows of dorsal and a pair of apical spurs. No glandular spurs on legs. Supra­anal plate as in Figure 3 E. Sex mean sd max min n Femur III F 10.7 0.4 11.3 9.8 26 Tibia III F 11 0.5 12.3 9.9 26 Body Length* Ovipositor F F 11.6 9.9 1.5 0.8 14.4 11.3 8.9 6.5 28 28 MedPL F 2 0.3 3.1 1.4 28 MaxPW F 3.3 0.6 3.8 0.3 28 MaxEW F 2.7 0.1 2.9 2.5 28 Femur III M 9.6 0.7 10.8 8 24 Tibia III M 10.2 0.7 11.6 8.9 24 Body Length* MedPL M M 10.3 1.7 1.9 0.2 13.4 2.1 7.1 1.4 18 24 MaxPW M 2.8 0.2 3.2 2.4 24 MaxEW M 2.4 0.1 2.6 2.1 24 Adult males. Head coloration distinctly brighter than pronotum and body color, in dorsal view ( Fig. 1 A). Both male forewings coriaceous, covering two thirds of abdomen, without visible hairs nor stridulatory vein. Dorsal venation in high relief ( Fig. 2 A). Right wing larger and generally folded upon left wing, the latter with less defined and slightly different venation. The lateral border of the wings bent downwards, in their costal and radial area. There is a bright yellowish strip at wing’s edges, which contrast with wing’s dark brownish coloration. Males with conspicuous and distinctive meso­ and metanotal glands, covered by the forewings ( Fig. 2 B). Subgenital plate longer than wide, with posterior median invagination, forming a pouch ( Fig. 3 C), that contains genitalia and spermatophore ( Fig. 3 F). Male genitalia. Male genitalia with lateral projections that bend dorsally to the posterior direction ( Fig. 3 G), and with a row of teeth in the dorsal face of their enlarged apices ( Fig. 2 C). Before extraction, these apices emerge laterally, one in each side, upon the subgenital plate. Sometimes each apex bears an amorphous tubular secretion extruded from its dorsal face ( Fig. 2 C). The projections present internal wrinkles, embracing a pair of complex median­posterior processes ( Fig. 2 D). These processes are large, highly sclerified and rounded structures, covered with a membranous layer in dorsum, with a rugose region in their posterior­lateral internal face. These processes present an unique ventral tooth ( Fig 2 E). In the basal region of these processes there is a group of intermingled sclerites, with a pair of clamp­like sclerites among them, dorsally visible, that may be hooked together ( Fig. 2 D, arrow). Ventrally, there is a group of median sclerites that project downwards, in the direction of the spermatophore. Some specimens may present distorted genitalia, apparently due to chemical interference in the clarification process. Adult females. Head coloration does not differ from pronotum and body color ( Fig. 1 B). Wingless, without meso­ nor metanotal glands. Dorsal coloration of abdomen dark, with pale spots restricted to a median region. Subgenital plate with posterior median invagination, wider than long. Bursa copulatrix (= copulatory papilla) without developed basal area, with obliquely truncated apex. In adults, ovipositor with distinctly widened apex, flattened laterally ( Fig. 1 B). Nymphs. Dorsal coloration of abdomen of both male and female nymphs dark, with pale spots restricted to a median region ( Fig. 2 F). Antennae color pattern as follows: scape and next five antennomeres dark, then one white, two dark, one white, four dark, two white, four dark, and two white. In sequence there is alternation of longer dark portions and shorter white ones. Female nymph’s ovipositor distinct from adult’s by being shorter, with pubescence along it’s entire length, and no enlarged apex. Chromosomes. Chromosome number is 2n = 11 in males ( Fig. 4 ), 2n = 12 in females, with a XO (male), XX (female) sex determining mechanism.