Bioacoustics of Phyllopalpus nicolaita

Description. Female. Body length: 9.2 ± 0.3 mm . Tegmina length : 7.2 ± 0.1 mm . Hind femur length : 5.9 ± 0.07 mm . Hind tibia length : 6.1 ± 0.06 mm . Ovipositor length : 3.3 ± 0.02 mm . Cercus length : 2.3 ± 0.09 mm . Abdomen length : 4.6 ± 0.19 mm . Color : Body black except frontal area, between compound eyes, with a red-orange spot; middle section of the antennae pale yellow, the rest dark; pronotum with lateral margins pale yellow; rest of the frons, clypeus, mandibles, and tip of the ovipositor shading from reddish-orange to dark brown; eyes silver; distal section of cerci pale yellow; hind wing light brown and translucent; legs shading from pale yellow to yellow; second tarsal segment and tarsal claw in all legs, as well as the ventral area of the first tarsal segment in both front and middle legs dark brown; abdominal tergites shading from brown to black and pleural membrane whitish. Head and pronotum finely punctate; head slightly shorter than the pronotum (length: 1.7 ± 0.03 mm vs. 2.1 ± 0.05 mm ) in dorsal view; head width from outer edge of eyes 2.4 ± 0.03 mm ; anterior width of pronotum subequal to posterior width (2.0 ± 0.03 mm / 2.1 mm ± 0.03 mm ); eyes slightly prominent not higher than vertex (in lateral view); antenna filiform with more than 208 antennomeres, very slender and longer than body (1.9 times); pronotum with lateral margins recurved; tegmina and hind wings extending beyond the apex of the abdomen, hind wings longer than tegmina. Male. Body length : 8.0 ± 0.48 mm . Head length : 1.6 ± 0.06 mm . Head width from outer edge of eyes : 2.2 ± 0.04 mm . Pronotum length : 1.8 ± 0.02 mm . Tegmina length : 6.3 ± 0.08 mm . Hind femur length : 5.1 ± 0.17 mm . Hind tibia length : 5.1 ± 0.15 mm . Cercus length : 1.6 ± 0.07 mm . Abdomen length : 4.1 ± 0.23 mm . Color: Similar to females but it’s tegmina with hyaline portions. The same color pattern as in females except the right tegmen is almost black and the left tegmen brown with a hyaline central portion including mirror, harp, basal area, and a part of the chordal area. Stridulatory file with 70–73 teeth. Antenna with more than 199 antennomeres and its length twice as long as body. DNA sequences. The fragments of the COI were successfully amplified for the specimens of P. nicolaita sp. nov. The COI sequences were 692 bp long. The obtained sequences of the mitochondrial gene of P. nicolaita sp. nov. proved to be sufficient to distinguish it from P. pulchellus , which is a unique species where a molecular characterization has been undertaken. The new sequence of P. nicolaita sp. nov. was deposited in GenBank (GenBank Acc. No. PQ179267). The COI of P. nicolaita sp. nov. was 93.61% similar to P. pulchellus (KF670975.1 and OR235176.1) from USA . The high similitude found in the same gene with P. pulchellus could indicate that this molecular marker separates the genus from other genera of the tribe Phylloscyrtini .