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What the sources say about this taxon, as written.
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The assembly and annotation data of Salvia miltiorrhiza Bunge (Lamiaceae) in the Genome Warehouse in BIG Data Center under Project numbers PRJCA003150, which are accessible at https://bigd.big.ac.cn/gwh ( Song et al., 2020 ). The Arabidopsis NAC amino acid sequences were obtained from TAIR (http://www.arabidopsis.org) and were used as query in searches against the S. miltiorrhiza genome database using the BLASTP program to obtain homologous sequences ( Jin et al., 2020 ). The Hidden Markov Model (HMM) corresponding to the NAC domain (PF02365) was downloaded from the pfam protein database, and HMMER 3.2 was used to examine the NAC genes from the BLASTP aligned sequences. Default parameters were employed, and the cutoff value was set to 0.01 ( Letunic and Bork 2018 ). Genes encoding proteins containing NAC domains were identified as NAC genes. All the Sm -NACs were mapped to the eight chromosomes and one scaffold of S. miltiorrhiza using the TBtools program and the physical locational information from the S. miltiorrhiza genome ( Li et al., 2020a ). A multi-sequence alignment of NAC proteins from Arabidopsis and S. miltiorrhiza was performed using ClustalW in MEGA7.0 with default parameters (https://www.megasoftware.net/). Because the Sm-NAC family sequence lengths varied greatly, the alignment results were used to construct a phylogenetic tree using the Maximum Likelihood method with 1000 bootstrap replicates ( Felsenstein 1985 ; Jones et al., 1992 ). Additionally, Evolview (http://www.evolgenius.info/) was used to beautify the evolutionary tree ( Subramanian et al., 2019 ). 5.2. Gene structure and protein motif analyses of Sm-NAC genes An online program of the gene structure display server (GSDS2.0) (http://gsds.cbi.pku.edu.cn/index.php) was used to draw the exonintron distribution of each Sm -NAC gene by comparing predicted coding sequences ( Hu et al., 2015 ). Conserved motifs of Sm-NAC protein sequences were investigated using the online software MEME5.0.4 (htt p://meme-suite.org/tools/meme) with default values for the motif parameters and the number of motifs searched set as 20 ( Bailey et al., 2009 ; Munir et al., 2020 ). TBtools was used to visualize the results ( Chen et al., 2020 ).