Bioacoustics of Distoseptispora aquatica

Notes. Distoseptispora nanchangensis was synonymized with D. aquatica by Shen et al. (2025 ) based on morphological and phylogenetic evidence. Distoseptispora longispora and D. zhejiangensis were introduced as new species solely on the basis of conidiophore and conidial size, despite minimal nucleotide divergence ( Song et al. 2020 ; Liao et al. 2025 a ). However, in the phylogenetic tree (Fig. 6 ), D. longispora was grouped with D. aquatica strains, consistent with the results of Liao et al. (2025 b ), whereas D. zhejiangensis was grouped with D. nanchangensis , consistent with Liao et al. (2025 a ). Distoseptispora longispora and D. zhejiangensis show conidiophore and conidial morphologies similar to those of D. aquatica , with short, cylindrical conidiophores and obclavate, brown to yellowish-brown conidia. While they differ only in conidial size and septation, they display minor molecular differences ( Song et al. 2020 ; Liao et al. 2025 a ; see Table 1 for details). Therefore, D. longispora and D. zhejiangensis are treated as synonyms of D. aquatica . Species Conidiophores Conidia ITS (bp) LSU (bp) TEF 1 - α (bp) RPB 2 (bp) D. aquatica ( MFLUCC 15-0374 ) Cylindrical, dark brown, 12–28 µm × 8–9.5 µm, 2–3 - septate Obclavate, elongated, dark brown with bluish green to malachite green tinge, 110–157 × 14–17, 15–28 - distoseptate – – – – D. longispora ( HFJAU 0705 ) Cylindrical, brown to dark brown, 17–37 x 6–10 um, 2–4 - septate Obclavate, elongated, brown to yellowish brown, 189–297 × 16–23 µm, 31–56 - distoseptate 5 / 503 (0.9 %) 6 / 834 (0.7 %) NA NA D. nanchangensis ( HJAUP C 1074 ) Cylindrical, brown to dark brown, 18–76 × 6–8 µm, 2–5 - septate Obclavate, brown to dark brown, 149–293 × 11–18 µm, (17 –) 21–43 - distoseptate 2 / 582 (0.3 %) 0 / 524 (0 %) 4 / 877 (0.4 %) 6 / 753 (0.7 %) D. zhejiangensis ( HJAUP C 2588 ) Cylindrical, brown, 16–56 × 13.5–20 µm, 1–3 - septate Obclavate, brown, 128–262 × 18.7–26.7 µm, 13–20 - distoseptate 2 / 587 (0.3 %) 1 / 543 (0.1 %) 4 / 877 (0.4 %) NA “ NA ” indicates the sequence unavailability.